Images

THE FOLLOWING CONSTRUCTS CAN BE REQUESTED

To view sequences click on the corresponding construct.

To view the literature, click on the reference.

MAPS & SEQUENCES mTangerine mHoneydew tdTomato dTomato	REFERENCES Shaner et.al
mCFP mYFP MyrPalm-CFP-pCDNA3 MyrPalm-YFP	Zacharias et.al.
Citrine YC2.3-pCDNA3 YC3.3	Griesbeck et.al.
Cameleon D1ER D1ER sequence	Palmer et.al.
mt Cameleon mt Cameleon Sequence	Palmer et.al.
AKAR1 and derivatives	Zhang et.al.
EGFR reporter Src reporter Ab1 reporter	Ting et.al.
mOrange2 (TXT) (PDF) TagRFP-T in pcDNA3 (TXT) (PDF) TagRFP-T in Bacterial Expression Vector (TXT) (PDF)	Shaner et. al.
roGFP	Not Available (see Jim Remington, Univ of Oregon)

THE FOLLOWING CONSTRUCTS ARE AVAILABLE FROM CLONTECH

Clontech

mCherry

mStrawberry

mOrange

mBanana

Shaner et.al

mPlum-pBAD

mRaspberry-pBAD

Wang et.al.

FILTER PAPER EXTRACTION PROCEDURE:

The enclosed filter paper holds about 1ug DNA. To extract the sample out of the filter paper, place the filter paper containing the DNA sample in a tube, pipet into the tube enough water or TE buffer to be absorbed and yield about 5-10 ul of excess liquid in the tube. Wait for 5 minutes then pipet out the liquid and transform into E.coli strain desired.